Anti-NMDAR1 Antibody [S308-48] (A304953)

Name: Anti-NMDAR1 Antibody [S308-48]
Brand: Antibodies.com
SKU: A304953
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S308-48] antibody to NMDAR1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Neuronal Marker

Specifications

Name

Anti-NMDAR1 Antibody [S308-48]

Description

Mouse monoclonal [S308-48] antibody to NMDAR1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to the extracellular N-terminus, amino acids 42-361, of rat NR1 protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

S308-48

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~105 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GluN1, Glutamate receptor ionotropic N methyl D aspartate 1, Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1, glutamate receptor ionotropic, NMDA 1, Glutamate [NMDA] receptor subunit zeta-1, Grin1, MRD8, N methyl D aspartate receptor, N methyl D aspartate receptor channel subunit zeta 1, N methyl D aspartate receptor subunit NR1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, NMDA 1, NMDA R1, NMDA receptor 1, NMDA1, NMDAR, NMDZ1_HUMAN, NR1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-NMDAR1 Antibody [S308-48] (A304953)

Western blot analysis of rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Anti-NMDAR1 Antibody [S308-48] (A304953) at 1:1,000.

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Figure 2: Immunofluorescence - Anti-NMDAR1 Antibody [S308-48] (A304953)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-NMDAR1 Antibody [S308-48] (A304953), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cell Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN1/NR1 Antibody. (D) Composite.

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Figure 3: Immunofluorescence - Anti-NMDAR1 Antibody [S308-48] (A304953)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-NMDAR1 Antibody [S308-48] (A304953), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN1/NR1 Antibody (D) Composite.

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