Anti-Nkx3.1 Antibody (A307522)

Name: Anti-Nkx3.1 Antibody
Brand: Antibodies.com
SKU: A307522
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Nkx3.1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Nkx3.1 Antibody

Description

Rabbit polyclonal antibody to Nkx3.1.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence within amino acids 1-80 of human NKX3.1 (NP_006158.2).

Sequence

MLRVPEPRPGEAKAEGAAPPTPSKPLTSFLIQDILRDGAQRQGGRTSSQRQRDPEPEPEPEPEGGRSRAGAQNDQLSTGP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

34 - 38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BAPX 2, BAPX2, Homeobox protein NK-3 homolog A, Homeobox protein Nkx 3.1, Homeobox protein Nkx-3.1, Homeobox protein Nkx3.1, NK homeobox (Drosophila) family 3 A, NK homeobox family 3 A, NK homeobox, family 3, member A, NK3 homeobox 1, NK3 transcription factor homolog A, NK3 transcription factor related locus 1, NKX 3, Nkx 3.1, NKX 3A, NKX3, NKX3 1, Nkx3-1, Nkx3.1, mouse, homolog of, NKX31_HUMAN, NKX3A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Nkx3.1 Antibody (A307522)

Western blot analysis of extracts of LNCaP cells, using Anti-Nkx3.1 Antibody (A307522) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Nkx3.1 Antibody (A307522)

Western blot analysis of extracts of Mouse kidney, using Anti-Nkx3.1 Antibody (A307522) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunofluorescence - Anti-Nkx3.1 Antibody (A307522)

Immunofluorescence analysis of LNCaP cells using Anti-Nkx3.1 Antibody (A307522) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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