Anti-Nkx2.5 Antibody (A83026)

Name: Anti-Nkx2.5 Antibody
Brand: Antibodies.com
SKU: A83026
Price: 480.0 USD
Availability: InStock

This product is identical to Abcam (ab106923). Discover more for less.

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$480

Goat polyclonal antibody to Nkx2.5 for ELISA, WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Nkx2.5 Antibody

Description

Goat polyclonal antibody to Nkx2.5.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:32,000, WB: 1-3µg/ml, IHC: 5-6µg/ml

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the internal region of human Nkx2.5.

Sequence

C-PRAYSDPDPAKDPR

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

38 kDa

Predicted MW

34.9kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cardiac-specific homeobox, Cardiac-specific homeobox 1, CHNG5, CSX, CSX1, FLJ52202, FLJ97166, FLJ97195, FLJ97197, FLJ99536, HLHS2, Homeobox protein CSX, Homeobox protein NK-2 homolog E, Homeobox protein Nkx 2.5, Homeobox protein Nkx-2.5, NK2 homeobox 5, NK2 transcription factor related locus 5, NK2 transcription factor related, locus 5 (Drosophila), NK2, Drosophila, homolog of, E, NKX2-5, NKX2.5, mouse, homolog of, NKX25_HUMAN, Nkx2E, NKX4-1, Tinman paralog, VSD3

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Nkx2.5 Antibody (A83026)

Anti-NKX2-5 Antibody (A83026) (0.3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-Nkx2.5 Antibody (A83026)

Anti-NKX2-5 Antibody (A83026) (3.75µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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Figure 3: Immunofluorescence - Anti-Nkx2.5 Antibody (A83026)

Anti-NKX2-5 Antibody (A83026) - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing nuclear and cytoplasmic staining.

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