Anti-NKCC1 Antibody (A84750)

Name: Anti-NKCC1 Antibody
Brand: Antibodies.com
SKU: A84750
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to NKCC1 for ELISA, IHC, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-NKCC1 Antibody

Description

Goat polyclonal antibody to NKCC1.

Applications

ELISA, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:64,000, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human NKCC1.

Sequence

C-NALQHSIRLSGVEDH

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Basolateral Na-K-Cl symporter, BSC, BSC2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, mBSC2, MGC104233, PPP1R141, Protein phosphatase 1, regulatory subunit 141, S12A2_HUMAN, Slc12a2, sodium-potassium-chloride cotransporter 1, Solute carrier family 12 (sodium/potassium/chloride transporter), member 2, solute carrier family 12 (sodium/potassium/chloride transporters) member 2, Solute carrier family 12 member 2, sy-ns

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NKCC1 Antibody (A84750) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle and membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Flow Cytometry - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-NKCC1 Antibody (A84750) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 3: Immunohistochemistry - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-NKCC1 Antibody (A84750) at 3.75µg/ml and revealed with alkaline phosphatase (AP).

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