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Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528)

Western Blot - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) - Antibodies.com Immunohistochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) - Antibodies.com Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Nicotinic Acetylcholine Receptor alpha 7 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody
Description
Rabbit polyclonal antibody to Nicotinic Acetylcholine Receptor alpha 7.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human CHRNA7 (NP_001177384.1).
Sequence
ASPPSTPPWDPGHIPGASVRPAPGPVSLQGEFQRKLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQHCKLKFGSWSYGGWSLDLQMQEADISGYIPNGEWDLVGI
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
53 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
7nAChR, a7 nicotinic acetylcholine receptor, acetylcholine receptor, neuronal nicotinic, alpha-7 subunit, ACHA7_HUMAN, AChR, Acra7, alpha 7 neuronal nicotinic acetylcholine receptor, alpha-7 nicotinic cholinergic receptor subunit, alpha7, cholinergic receptor, neuronal nicotinic, alpha polypeptide 7, cholinergic receptor, nicotinic, alpha 7, cholinergic receptor, nicotinic, alpha 7 (neuronal), cholinergic receptor, nicotinic, alpha polypeptide 7, CHRNA7, CHRNA7-2, NACHRA7, neuronal acetylcholine receptor protein, alpha-7 chain, Neuronal acetylcholine receptor subunit alpha-7
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528)
Western blot analysis of extracts of various cell lines, using Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528)
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528)
Immunofluorescence analysis of L929 cells using Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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