Rabbit polyclonal antibody to NFκB-p65 (Phospho-Ser276)
Specificity
The antibody detects endogenous level of NFκB-p65 only when phosphorylated at serine 276.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500-1:1000
Reactivity
Human, Mouse, Rat
Immunogen
: Peptide sequence around phosphorylation site of serine 276(R-P-S(p)-D-R) derived from Human NFκB-p65.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extract from Hela cells using NF-κB p65 (phospho-Ser276) antibody #11011. Lane 1: The antibody is not preincubated with blocking peptides. Lane 2: The antibody is preincubated with non- phospho peptide blocking peptides #61011. Lane 3: The antibody is preincubated with phospho peptide blocking peptides #51011.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) Antibody #11011 (left) or the same antibody preincubated with blocking peptide #51011 (right).
