Anti-NF2 / Merlin Antibody (A93014)

Name: Anti-NF2 / Merlin Antibody
Brand: Antibodies.com
SKU: A93014
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit polyclonal antibody to NF2 / Merlin for WB, ICC/IF and IP.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-NF2 / Merlin Antibody

Description

Rabbit polyclonal antibody to NF2 / Merlin.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2 (NP_000259.1).

Sequence

TKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ACN, BANF, Bilateral acoustic neuroma, Merlin, MERL_HUMAN, Moesin ezrin radixin like protein, Moesin ezrin radizin like, Moesin-ezrin-radixin-like protein, Neurofibromatosis 2, Neurofibromatosis type 2, Neurofibromatosis2, Neurofibromin 2, Neurofibromin-2, Neurofibromin2, NF 2, Nf2, SCH, Schwannomerlin, Schwannomin

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

Enlarge Image

Figure 1: Western Blot - Anti-NF2 / Merlin Antibody (A93014)

Western blot analysis of extracts of various cell lines, using Anti-NF2 / Merlin Antibody (A93014) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Enlarge Image

Figure 2: Western Blot - Anti-NF2 / Merlin Antibody (A93014)

Western blot analysis of extracts from normal (control) and NF2 knockout (KO) HeLa cells, using Anti-NF2 / Merlin Antibody (A93014) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 6s.

Enlarge Image

Figure 3: Immunofluorescence - Anti-NF2 / Merlin Antibody (A93014)

Immunofluorescence analysis of C6 cells using Anti-NF2 / Merlin Antibody (A93014) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 4: Immunofluorescence - Anti-NF2 / Merlin Antibody (A93014)

Immunofluorescence analysis of L929 cells using Anti-NF2 / Merlin Antibody (A93014) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).