Western blot analysis of extracts of various cell lines, using Anti-NF2/Merlin Antibody (A93014) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western blot analysis of extracts from normal (control) and NF2 knockout (KO) HeLa cells, using Anti-NF2/Merlin Antibody (A93014) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 6s.
Immunofluorescence analysis of L929 cells using Anti-NF2/Merlin Antibody (A93014) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-NF2/Merlin Antibody (A93014) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 200µg extracts of SKOV3 cells using 3µg of Anti-NF2/Merlin Antibody (A93014). This Western blot was performed on the immunoprecipitate using Anti-NF2/Merlin Antibody (A93014) at a dilution of 1:1000.
