Anti-NF-M Antibody (A85324)

Name: Anti-NF-M Antibody
Brand: Antibodies.com
SKU: A85324
Price: 185.0 USD
Availability: InStock

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$185

Chicken polyclonal antibody to NF-M for WB, ICC/IF and IHC.

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Highlighted products for Anti-NF-M Antibody

(4)

A85323

1 Citation

Anti-NF-M Antibody

Rabbit polyclonal antibody to NF-M for WB, ICC/IF and IHC.

(4)

A85325

1 Citation

Anti-NF-M Antibody [3H11]

Mouse monoclonal (3H11) antibody to NF-M for WB, ICC/IF and IHC.

(4)

A85323

1 Citation

Anti-NF-M Antibody

Rabbit polyclonal antibody to NF-M for WB, ICC/IF and IHC.

(4)

A85325

1 Citation

Anti-NF-M Antibody [3H11]

Mouse monoclonal (3H11) antibody to NF-M for WB, ICC/IF and IHC.

Specifications

Name

Anti-NF-M Antibody

Description

Chicken polyclonal antibody to NF-M.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:2,000-1:5,000, ICC/IF: 1:500-1:1,000, IHC: 1:500-1:1,000

Reactivity

Human, Horse, Cow, Porcine, Chicken, Rat, Mouse

Immunogen

Recombinant construct containing the C-terminus, amino acids 708-877, of human NF-M, expressed in and purified from E. coli.

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Conjugate

Unconjugated

Concentration

20-30 mg/ml

Molecular Weight

145-160 kDa (by SDS-PAGE)

Purity

IgY preparation.

Product Form

Liquid

Formulation

Supplied as an aliquot of IgY preparation with 5mM Sodium Azide.

Synonyms

150kDa medium, 160 kDa neurofilament protein, 2 kDa neurofilament protein, 200 kDa neurofilament protein, 68 kDa neurofilament protein, AI847934, CMT1F, CMT2CC, CMT2E, FLJ53642, KIAA0845, light molecular weight neurofilament protein, Micro glutamic acid-rich protein, mKIAA0845, Nef3, NEFH, NEFL, NEFM, Neurofilament, Neurofilament 3, Neurofilament heavy polypeptide, Neurofilament heavy polypeptide 200kDa, Neurofilament light polypeptide, Neurofilament light polypeptide 68kDa, Neurofilament medium polypeptide, Neurofilament medium polypeptide 150kDa, Neurofilament protein heavy polypeptide, Neurofilament protein light chain, Neurofilament protein light polypeptide, Neurofilament protein medium polypeptide, neurofilament subunit NF-L, Neurofilament triplet H protein, Neurofilament triplet L protein, Neurofilament triplet M protein, neurofilament-3 (150 kD medium), NF-H, NF-L, NF160, NF165, NF200, NF68, NFH, NFL, NFM, NFM_HUMAN, PPP1R110, protein phosphatase 1, regulatory subunit 110

Isotype Controls

Chicken IgY (A63673)

Suitable Secondaries

See all Anti-Chicken IgY Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunofluorescence - Anti-NF-M Antibody (A85324)

Immunofluorescent analysis of rat cerebellum section stained with Anti-NF-M Antibody (1:1,000 | red) and Anti-CNPase Antibody (A85413 | 1:500 | green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free floating sections were stained with the above antibodies. The Anti-NF-M Antibody labels the axons of basket and other neurons, while the Anti-CNPase Antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.

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Figure 2: Immunofluorescence - Anti-NF-M Antibody (A85324)

Immunofluorescent analysis of rat neuron/glial cell cultures stained with Anti-NF-M Antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites, and perikarya of neurons. The blue is DAPI staining of nuclear DNA.

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Figure 3: Western Blot - Anti-NF-M Antibody (A85324)

Western blot analysis of different neuronal tissue and cell lysates using Anti-NF-M Antibody (1:2,000 | green): [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.

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Figure 4: Western Blot - Anti-NF-M Antibody (A85324)

Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with Anti-NF-M Antibody which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes - now a ~100kDa band is seen in addition to the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.

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