Anti-NF-kB p65 Antibody (A283785)

Anti-NF-kB p65 Antibody

Rabbit polyclonal antibody to NF-kB p65.

This antibody recognises the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NF?B p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NF?B p65 is a 551 amino acid ~65 kDa subunit of the NF?B transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NF?B appears to be the p65-p50 heterodimer. In the active form NF?B p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase DDX1. NF?B p65 is also found in an inactive form in the cytoplasm associated with the inhibitor I?B (UniProt: Q04206) In a gel supershift assay Rabbit anti Human NF?B p65 antibody was found to be active against all p65 containing human, mouse or rat NF?B complexes using 0.5ul to 1.0ul per assay. The binding of Rabbit anti Human NF?B p65 antibody to the NF?B p65 subunit can be blocked using the NF?B p65 control peptide (PHP073).

WB, ELISA, IF, IP, Gel Super Shift Assays

ELISA: 1:5,000 - 1:25,000, Gel Super Shift Assays: 0.5 - 1 ul per test, WB: 1:2,000 - 1:5,000

Human, Mouse, Rat

NFkB p65 peptide corresponding to the C-terminus region of the human protein conjugated to Keyhole Limpet Haemocyanin.

Rabbit

Polyclonal

IgG

Unconjugated

Lot Specific

Liquid

Supplied as serum with 0.01% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Human NFkB p65 antibody recognizes the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NF?B p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NF?B p65 is a 551 amino acid ~65 kDa subunit of the NF?B transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NF?B appears to be the p65-p50 heterodimer. In the active form NF?B p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase DDX1. NF?B p65 is also found in an inactive form in the cytoplasm associated with the inhibitor I?B (UniProt: Q04206) In a gel supershift assay Rabbit anti Human NF?B p65 antibody was found to be active against all p65 containing human, mouse or rat NF?B complexes using 0.5ul to 1.0ul per assay. The binding of Rabbit anti Human NF?B p65 antibody to the NF?B p65 subunit can be blocked using the NF?B p65 control peptide (PHP073).

Avian reticuloendotheliosis viral (v rel) oncogene homolog A, MGC131774, NF kappa B p65delta3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, OTTHUMP00000233473, OTTHUMP00000233474, OTTHUMP00000233475, OTTHUMP00000233476, OTTHUMP00000233900, p65, p65 NF kappaB, p65 NFkB, relA, TF65_HUMAN, Transcription factor p65, V rel avian reticuloendotheliosis viral oncogene homolog A, v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)), v rel reticuloendotheliosis viral oncogene homolog A (avian), V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65