Anti-Neuroserpin Antibody (A83659)

Neuroserpin expression in Kelly (A) and K562 (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Neuroserpin Antibody (A83659) at 1µg/ml and detected by chemiluminescence.

Neuroserpin expression in Human Placenta (A), Mouse Brain (B), and Rat Braun (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-Neuroserpin Antibody (A83659) at 1µg/ml (A-B) or 0.5µg/ml (C) and detected by chemiluminescence.

Neuroserpin expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Neuroserpin Antibody (A83659) at 5µg/ml and revealed with alkaline phosphatase (AP).

Neuroserpin expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Neuroserpin Antibody (A83659) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.