Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free (A278318)

Name: Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free
Brand: Antibodies.com
SKU: A278318
Price: 525.0 USD
Availability: InStock

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$525

Mouse monoclonal [PCRP-NEUROG3-1E10] antibody to NeuroG3 for ELISA, IP and Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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+1 (314) 370-6046

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Monospecific Antibody

Specifications

Name

Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free

Description

Mouse monoclonal [PCRP-NEUROG3-1E10] antibody to NeuroG3.

Specificity

This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1.The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.

Applications

ELISA, IP, Flow Cytometry

Dilutions

IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells

Reactivity

Human

Immunogen

Recombinant full-length human NeuroG3 protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

PCRP-NEUROG3-1E10

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Protein A/G chromatography.

Concentration

1 mg/ml

Molecular Weight

23.54 kDa

Product Form

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] (A277730).

Synonyms

ATH4B, Atoh5, bHLHa7, Class A basic helix-loop-helix protein 7, Math4B, Neurogenin-3, Neurogenin3, NGN-3, ngn3, NGN3_HUMAN, OTTHUMP00000019730, Protein atonal homolog 5

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Flow Cytometry - Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free (A278318)

Flow cytometric analysis of PFA-fixed HeLa cells using Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 2: Immunofluorescence - Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free (A278318)

Immunofluorescent analysis of PFA-fixed HeLa cells stained with Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640A Phalloidin (Red).

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Figure 3: SDS-PAGE - Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free (A278318)

SDS-PAGE analysis of Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Figure 4: Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free (A278318)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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