Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] (A277730)

Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10]

Mouse monoclonal [PCRP-NEUROG3-1E10] antibody to NeuroG3.

This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1.The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.

ELISA, IP, Flow Cytometry

IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells

Human

Recombinant full-length human NeuroG3 protein.

Mouse

Monoclonal

PCRP-NEUROG3-1E10

IgG2b

Protein A/G chromatography.

200 µg/ml

23.54 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-NeuroG3 Antibody [PCRP-NEUROG3-1E10] - BSA and Azide free (A278318).

ATH4B, Atoh5, bHLHa7, Class A basic helix-loop-helix protein 7, Math4B, Neurogenin-3, Neurogenin3, NGN-3, ngn3, NGN3_HUMAN, OTTHUMP00000019730, Protein atonal homolog 5