Anti-Neurexin 1 Antibody (A83333)

Neurexin 1 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress FLAG tagged Neurexin 1 an lysed in RIPA buffer. Primary incubation was performed on 10µg protein per lane with Anti-Neurexin 1 Antibody (A83333) at or Anti-FLAG at 1:3000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-Neurexin 1 Antibody (A83333) at 1mg/ml (Lane B).

Neurexin 1 expression in Kelly cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Neurexin 1 Antibody (A83333) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.