Anti-NEDD8 Antibody (A88225)

Name: Anti-NEDD8 Antibody
Brand: Antibodies.com
SKU: A88225

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This product is discontinued

Anti-NEDD8 Antibody (A88225) has been discontinued and is no longer available.

View all NEDD8 Antibodies.

Specifications

Name

Anti-NEDD8 Antibody

Description

Rabbit polyclonal antibody to NEDD8.

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NEDD8 (NP_006147.1).

Sequence

MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLRQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

13 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

NEDD-8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein Nedd8

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-NEDD8 Antibody (A88225)

Western blot analysis of extracts of various cell lines, using Anti-NEDD8 Antibody (A88225) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.