Anti-NEDD4-2 (phospho Ser448) Antibody (A88143)

Name: Anti-NEDD4-2 (phospho Ser448) Antibody
Brand: Antibodies.com
SKU: A88143
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to NEDD4-2 (phospho Ser448) for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-NEDD4-2 (phospho Ser448) Antibody

Description

Rabbit polyclonal antibody to NEDD4-2 (phospho Ser448).

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S448 of human NEDD4L (NP_001138439.1).

Sequence

SLSSP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa / 130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E3 ubiquitin protein ligase NEDD4 like protein, E3 ubiquitin-protein ligase NEDD4-like, KIAA0439, NED4L_HUMAN, NEDD4 2, NEDD4 2c, NEDD4-2a, NEDD4.2, NEDD4b, NEDD4L, NEDD4La, NEDD4Lb, NEDD4Lc, NEDD4Ld, NEDD4Le, NEDD4Lf, NEDD4Lg, NEDD4Lh, NEDL3, Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase, Neural precursor cell expressed, developmentally down regulated 4 like, RSP5, Ubiquitin protein ligase Rsp5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-NEDD4-2 (phospho Ser448) Antibody (A88143)

Western blot analysis of extracts of various cell lines, using Anti-NEDD4-2 (phospho Ser448) Antibody (A88143) at 1:1,000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-NEDD4-2 (phospho Ser448) Antibody (A88143)

Western blot analysis of extracts of 293T cells, using Anti-NEDD4-2 (phospho Ser448) Antibody (A88143) at 1:1,000 dilution or NEDD4L antibody (A8085). 293T cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 5s.

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