Anti-Ndufs4 Antibody [ARC1784] (A306828)

Name: Anti-Ndufs4 Antibody [ARC1784]
Brand: Antibodies.com
SKU: A306828
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1784] antibody to Ndufs4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Ndufs4 Antibody [ARC1784]

Description

Rabbit monoclonal [ARC1784] antibody to Ndufs4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NDUFS4 (O43181).

Sequence

MAAVSMSVVLRQTLWRRRAVAVAALSVSRVPTRSLRTSTWRLAQDQTQDTQLITVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKME

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1784

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

19 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AQDQ, CI 18, CI 18 kDa, CI AQDQ, CI-18 kDa, CI-AQDQ, Complex I 18 kDa, Complex I AQDQ, Complex I-18 kDa, Complex I-AQDQ, mitochondrial, mitochondrial respiratory chain complex I (18 KD subunit), NADH coenzyme Q reductase, NADH dehydrogenase, NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa, NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, NADH ubiquinone oxidoreductase 18 kDa subunit, NADH-ubiquinone oxidoreductase 18 kDa subunit, NDUS4_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Ndufs4 Antibody [ARC1784] (A306828)

Western blot analysis of extracts of various cell lines, using Anti-Ndufs4 Antibody [ARC1784] (A306828) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Ndufs4 Antibody [ARC1784] (A306828)

Western blot analysis of extracts of HepG2 cells, using Anti-Ndufs4 Antibody [ARC1784] (A306828) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-Ndufs4 Antibody [ARC1784] (A306828)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Ndufs4 Antibody [ARC1784] (A306828) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Ndufs4 Antibody [ARC1784] (A306828)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Ndufs4 Antibody [ARC1784] (A306828) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.