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Anti-NDUFB4 Antibody (A92828)

Western Blot - Anti-NDUFB4 Antibody (A92828) - Antibodies.com Western Blot - Anti-NDUFB4 Antibody (A92828) - Antibodies.com Immunohistochemistry - Anti-NDUFB4 Antibody (A92828) - Antibodies.com Immunohistochemistry - Anti-NDUFB4 Antibody (A92828) - Antibodies.com Immunohistochemistry - Anti-NDUFB4 Antibody (A92828) - Antibodies.com Immunofluorescence - Anti-NDUFB4 Antibody (A92828) - Antibodies.com Immunofluorescence - Anti-NDUFB4 Antibody (A92828) - Antibodies.com
Knockout Validated
$470
Product Datasheet

Rabbit polyclonal antibody to NDUFB4 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-NDUFB4 Antibody
Description
Rabbit polyclonal antibody to NDUFB4.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).
Sequence
MSFPKYKPSSLRTLPETLDPAEYNISPETRRAQAERLAIRAQLKREYLLQYNDPNRRGLIENPALLRWAYARTINVYPNFRPTPKNSLMG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
15 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
B15, CI-B15, Complex I B15 subunit, Complex I-B15, NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4 15kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NADH ubiquinone oxidoreductase B15 subunit, NADH-ubiquinone oxidoreductase B15 subunit, NDUB4_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-NDUFB4 Antibody (A92828) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-NDUFB4 Antibody (A92828)
Western blot analysis of extracts of various cell lines, using Anti-NDUFB4 Antibody (A92828) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-NDUFB4 Antibody (A92828) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-NDUFB4 Antibody (A92828)
Western blot analysis of extracts from normal (control) and NDUFB4 knockout (KO) HeLa cells, using Anti-NDUFB4 Antibody (A92828) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-NDUFB4 Antibody (A92828) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-NDUFB4 Antibody (A92828)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-NDUFB4 Antibody (A92828) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-NDUFB4 Antibody (A92828) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-NDUFB4 Antibody (A92828)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-NDUFB4 Antibody (A92828) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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