Western blot analysis of extracts of various cell lines, using Anti-NDUFA8 Antibody (A80741) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of H9C2 cells using Anti-NDUFA8 Antibody (A80741) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of L929 cells using Anti-NDUFA8 Antibody (A80741) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 200µg extracts of 293T cells using 3µg of Anti-NDUFA8 Antibody (A80741). This Western blot was performed on the immunoprecipitate using Anti-NDUFA8 Antibody (A80741) at a dilution of 1:1000.
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