Figure 1: Western Blot - Anti-NCF1/p47-phox Antibody (A83765)
NCF1/p47-phox expression in U937 (A), Daudi (B), U251 (C), and negative control A431 (D) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-NCF1/p47-phox Antibody (A83765) at 0.2µg/ml (A), 0.01µg/ml (B), or 0.3µg/ml (C-D) and detected by chemiluminescence.
Figure 2: Western Blot - Anti-NCF1/p47-phox Antibody (A83765)
NCF1/p47-phox expression in Mouse Thymus (A) and Pig Spleen (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-NCF1/p47-phox Antibody (A83765) at 1µg/ml and detected by chemiluminescence.
NCF1/p47-phox expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NCF1/p47-phox Antibody (A83765) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
NCF1/p47-phox expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-NCF1/p47-phox Antibody (A83765) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
NCF1/p47-phox expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-NCF1/p47-phox Antibody (A83765) at 5µg/ml and revealed with alkaline phosphatase (AP).
