This NAD(H) Assay Kit is a quantitative assay kit for measuring NAD/NADH in animal tissues, plant tissues, cells, bacteria, serum, and plasma.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
This kit uses a WST-8 based chromogenic reaction that uses colorimetry to measure the amounts, ratio, and total amount of NAD+ and NADH in cells, tissues, or other samples. The NAD+/NADH assay kit is based on an enzyme cycle reaction (which does not recognize NADP+/NADPH) in which NAD+ is reduced to NADH and NADH reduces WST-8 to an orange formazan with a maximum absorption peak detected at around 450 nm. The formation of formazan in the reaction system is proportional to the total amount of NAD+ or NADH in the sample.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, plant tissues, cells, bacteria, serum, and plasma.
Components
Assay Buffer, EtOH Solution, WST-8 Solution, Enhancer, NAD Cycling Enzyme Mix, NAD Standard (10 mM), NAD Extraction Buffer, NADH Extraction Buffer
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
96-well microplate / microglass cuvette is required.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
