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Anti-N6-Methyladenosine / m6A Antibody [ARC5003-10] (A309557)

Dot Blot - Anti-N6-Methyladenosine / m6A Antibody [ARC5003-10] (A309557) - Antibodies.com Immunofluorescence - Anti-N6-methyladenosine / m6A Antibody [ARC5003-10] (A309557) - Antibodies.com RNA Immunoprecipitation - Anti-N6-methyladenosine / m6A Antibody [ARC5003-10] (A309557) - Antibodies.com
$540
Product Datasheet

Rabbit monoclonal [ARC5003-10] antibody to N6-Methyladenosine / m6A for ICC/IF, IP, RIP, Nucleotide Array and Dot Blot.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-N6-Methyladenosine / m6A Antibody [ARC5003-10]
Description
Rabbit monoclonal [ARC5003-10] antibody to N6-Methyladenosine / m6A.
Applications
ICC/IF, IP, RIP, Nucleotide Array, Dot Blot
Dilutions
DB: 1:500-1:2,000, meRIP: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Species Independent
Immunogen
Chemical compounds corresponding to N6-methyladenosine / m6A.
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC5003-10
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A/G purification.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Dot Blot - Anti-N6-Methyladenosine / m6A Antibody [ARC5003-10] (A309557) - Antibodies.comEnlarge Image
Figure 1: Dot Blot - Anti-N6-Methyladenosine / m6A Antibody [ARC5003-10] (A309557)
Anti-N6-Methyladenosine / m6A Antibody [ARC5003-10] (A309557) was tested in dot blot against N6-methyladenosine (m6A) and unmodified adenosine. Oligomer 8: ATAACTGG-m6A-CCGAATGG Oligomer 9: ATAACTGGACCGAATGG Oligomer 10: AAAAAAAAAAAAAAAA-biotin.
Immunofluorescence - Anti-N6-methyladenosine / m6A Antibody [ARC5003-10] (A309557) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-N6-Methyladenosine / m6A Antibody [ARC5003-10] (A309557)
Immunofluorescence analysis of U-2 OS treated with UV(30s)Brdu(0min) and U-2 OS treated with UV(30s)Brdu(10min) cells using Anti-N6-methyladenosine / m6A Antibody [ARC5003-10] (A309557) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
RNA Immunoprecipitation - Anti-N6-methyladenosine / m6A Antibody [ARC5003-10] (A309557) - Antibodies.comEnlarge Image
Figure 3: Immunoprecipitation - Anti-N6-Methyladenosine / m6A Antibody [ARC5003-10] (A309557)
RNA Immunoprecipitation was performed on 100 µg mouse liver total RNA, using 5 µg of Anti-N6-methyladenosine / m6A Antibody [ARC5003-10] (A309557). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was verified by using HS6ST1 as PCR primer of qRT-PCR. The picture shows the relative enrichment multiple of HS6ST1 site.
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