The Anti-N6-Methyladenosine Antibody (A91811), (4µg) was tested in a Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer). Oligomer 1 - N6-methyladenosine (m6A-CUGGUAACGAAUGGCU-G-Biotin, Oligomer 2 - unmodified adenosine (ACUGGUAACGAAUGGCU-G-Biotin), Oligomer 3 - unmodified adenosine (AAAAAAAAAAAAAAAAA-Biotin)
Figure 3: Dot Blot - Anti-N6-Methyladenosine Antibody (A91811)
Anti-N6-Methyladenosine Antibody (A91811) was tested in dot blot against N6-methyladenosine (m6A) and unmodified adenosine. Oligomer 8: ATAACTGG-m6A-CCGAATGG Oligomer 9: ATAACTGGACCGAATGG Oligomer 10: AAAAAAAAAAAAAAAA-biotin.
Immunofluorescence analysis of U2OS cells using Anti-N6-Methyladenosine Antibody (A91811). U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time. DAPI, 4', 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.
Immunoprecipitation analysis of 25µg total RNA extracts of Arabidopsis using 2ul Anti-N6-Methyladenosine Antibody (A91811). 25µg of total RNA extracts of Arabidopsis was diluted to 200 µl IP buffer system.
