Anti-N Cadherin Aptamer [NC3S] (Biotin) (A321195)

Freight Charges
Lead Time
30-45 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-N Cadherin Aptamer [NC3S] (Biotin)
Description
Synthetic DNA aptamer to N Cadherin [NC3S] (Biotin).
Aptamer ID
NC3S
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Dulbecco’s Phosphate Buffered Saline (without Calcium and Magnesium), with 4.5g/l Glucose, 5mM Magnesium Chloride, Yeast tRNA, and Bovine Serum Albumin. Binding Temperature: 4°C., 25°C, 37°C.
Sequence
5’-dTpdTpdGpdCpdApdCpdTpdApdTpdGpdTpdTpdTpdTpdApdGpdCpdTpdApdGpdGpdGpdTpdTpdCpdCpdCpdTpdCpdCpdGpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdAp-3’
Aptamer Length
47 nt
Aptamer Molecular Weight (Da)
14,499.4 Da
Aptamer Affinity
Affinity (Kd) = 20.08±0.72 nM
Extinction Coefficient
454400 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-N Cadherin Aptamer [NC3S] (Biotin) (A321195) is a replica of the aptamer described in PMID: 33174555.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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