Anti-N Cadherin Antibody [ARC0371] (A305345)

Name: Anti-N Cadherin Antibody [ARC0371]
Brand: Antibodies.com
SKU: A305345
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0371] antibody to N Cadherin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody Intercellular Junction Marker

Specifications

Name

Anti-N Cadherin Antibody [ARC0371]

Description

Rabbit monoclonal [ARC0371] antibody to N Cadherin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human N Cadherin (P19022).

Sequence

AKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0371

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CADH2_HUMAN, Cadherin 2, Cadherin 2 N cadherin neuronal, Cadherin 2 type 1, Cadherin 2 type 1 N cadherin neuronal, Cadherin 2, type 1, N-cadherin (neuronal), Cadherin-2, Cadherin2, Calcium dependent adhesion protein neuronal, CD325, CD325 antigen, CDH2, CDHN, CDw325, CDw325 antigen, N cadherin 1, N-cadherin, NCAD, Neural cadherin, OTTHUMP00000066304, OTTHUMP00000067378

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-N Cadherin Antibody [ARC0371] (A305345)

Western blot analysis of extracts from wild type (WT) and N-Cadherin knockout (KO) HeLa cells, using Anti-N Cadherin Antibody [ARC0371] (A305345) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 2: Western Blot - Anti-N Cadherin Antibody [ARC0371] (A305345)

Western blot analysis of extracts of various cell lines, using Anti-N Cadherin Antibody [ARC0371] (A305345) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 3: Immunohistochemistry - Anti-N Cadherin Antibody [ARC0371] (A305345)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-N Cadherin Antibody [ARC0371] (A305345) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-N Cadherin Antibody [ARC0371] (A305345)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-N Cadherin Antibody [ARC0371] (A305345) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.