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Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody (A93722)

Immunofluorescence - Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody (A0690) - Antibodies.com Immunohistochemistry - Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody (A0690) - Antibodies.com Western Blot - Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody (A0690) - Antibodies.com
$185
Product Datasheet

Rabbit polyclonal antibody to Myosin regulatory light chain 2 (phospho Ser18) for WB, IHC, IF and ELISA.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody
Description
Rabbit polyclonal antibody to Myosin regulatory light chain 2 (phospho Ser18).
Specificity
This antibody detects endogenous levels of Myosin regulatory light chain 2 only when phosphorylated at Ser18.
Applications
WB, IHC, IF, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Myosin regulatory light chain 2 around the phosphorylation site of Ser18 (amino acids 3-52).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
19kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
20 kDa myosin light chain, KMLC, LC20, MLC-2C, MLCK2, MRLC1, MYL9, MYL9_HUMAN, Myosin regulatory light chain 2, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, Myosin regulatory light polypeptide 9, Myosin RLC, Myosin, light chain 9, regulatory, MYRL2, smooth muscle isoform
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunofluorescence - Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody (A0690) - Antibodies.comEnlarge Image
Figure 1: Immunofluorescence - Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody (A93722)
Immunofluorescence analysis of HUVEC cells using Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemistry - Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody (A0690) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody (A93722)
Immunohistochemical analysis of paraffin-embedded human brain using Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody (A0690) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody (A93722)
Western blot analysis of lysates from LOVO cells treated with H2O2 100uM 30' using Anti-Myosin regulatory light chain 2 (phospho Ser18) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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