Anti-MyoD1 Antibody [SPM427] (A249465)

Mouse monoclonal [SPM427] antibody to MyoD1 for Flow Cytometry, IF and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-MyoD1 Antibody [SPM427]
Description
Mouse monoclonal [SPM427] antibody to MyoD1.
Specificity
This antibody recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms sarcomas and alveolar soft part sarcomas.
Applications
Flow Cytometry, IF, IHC-P
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Mouse, Rat, Chicken
Immunogen
Recombinant mouse MyoD protein.
Epitope
a.a. 180-189
Host
Mouse
Clonality
Monoclonal
Clone ID
SPM427
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
45 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-MyoD1 Antibody [SPM427] - BSA and Azide free (A252645).
Synonyms
bHLHc1, Class C basic helix-loop-helix protein 1, MYF 3, Myf-3, MYF3, Myoblast determination protein 1, MYOD, Myod 1, MYOD1_HUMAN, Myogenic differentiation 1, Myogenic factor 3, Myogenic factor MYF 3, Myogenin D1, PUM
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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