Anti-MYL9 (phospho Thr18 + Ser19) Antibody (A305466)

Name: Anti-MYL9 (phospho Thr18 + Ser19) Antibody
Brand: Antibodies.com
SKU: A305466
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to MYL9 (phospho Thr18 + Ser19) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-MYL9 (phospho Thr18 + Ser19) Antibody

Description

Rabbit polyclonal antibody to MYL9 (phospho Thr18 + Ser19).

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T18 & S19 of human MYL9 (NP_006088.2).

Sequence

ATSNV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

18 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

20 kDa myosin light chain, Human 20kDa myosin light chain (MLC2) mRNA complete cds, LC20, MGC3505, MLC 2, MLC-2C, MLC2, MLY 9, MRLC1, MYL9_HUMAN, Myosin light chain 9 regulatory, Myosin light polypeptide 9 regulatory, myosin regulatory light chain 1, Myosin regulatory light chain 2, Myosin regulatory light chain 2 smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, Myosin regulatory light polypeptide 9, Myosin RLC, Myosin vascular smooth muscle light chain 2, MYRL2, OTTHUMP00000030857, smooth muscle isoform

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-MYL9 (phospho Thr18 + Ser19) Antibody (A305466)

Western blot analysis of HeLa, using Anti-MYL9 (phospho Thr18 + Ser19) Antibody (A305466) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-MYL9 (phospho Thr18 + Ser19) Antibody (A305466)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-MYL9 (phospho Thr18 + Ser19) Antibody (A305466) at a dilution of 1:100 (20x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-MYL9 (phospho Thr18 + Ser19) Antibody (A305466)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-MYL9 (phospho Thr18 + Ser19) Antibody (A305466) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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