Recombinant rabbit monoclonal [MPO/33R] antibody to Myeloperoxidase.
Specificity
Myeloperoxidase (MPO) also called the peroxidase (POD), is an important marker of bone marrow cells. It is one of the members of the family of heme peroxidase super existing in myeloid cells (mainly neutrophils and monocytes of aniline blue particles). With the deepening of the research on MPO, MPO gene polymorphism has been found to lead to individual for some disease susceptibility differences, with a variety of human development is closely related to the occurrence of diseases. The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow. The antibody is useful as an aid for classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma.
Applications
WB, IHC-P
Dilutions
WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment, around amino acids 150-250, of human Myeloperoxidase. The exact sequence is proprietary.
Host
Rabbit
Clonality
Monoclonal
Clone ID
MPO/33R
Isotype
IgG
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
heavy-light promoter: 72 kDa / dimer: 140 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue using Anti-Myeloperoxidase Antibody [MPO/33R]. Inset: PBS instead of the primary antibody. Secondary antibody negative control.
Figure 3: Western Blot - Anti-Myeloperoxidase Antibody [MPO/33R] (A278030)
Western blot analysis of human spleen tissue lysate using Anti-Myeloperoxidase Antibody [MPO/33R].
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