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Anti-Myelin Basic Protein Antibody [ARC0535] (A80785)

Western Blot - Anti-Myelin Basic Protein Antibody [ARC0535] (A80785) - Antibodies.com Immunofluorescence - Anti-Myelin Basic Protein Antibody [ARC0535] (A80785) - Antibodies.com Immunofluorescence - Anti-Myelin Basic Protein Antibody [ARC0535] (A80785) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0535] antibody to Myelin Basic Protein for WB and ICC/IF.

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Anti-Myelin Basic Protein Antibody [7D2]
Mouse monoclonal (7D2) antibody to Myelin Basic Protein for WB, ICC/IF and IHC.
Name
Anti-Myelin Basic Protein Antibody [ARC0535]
Description
Rabbit monoclonal [ARC0535] antibody to Myelin Basic Protein.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 161-304 of human Myelin Basic Protein (P02686).
Sequence
GFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARR
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0535
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
14 - 23 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
GDB, Golli MBP, Golli MBP; myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MBP, MBP_CAVPO, MBP_HUMAN, MGC99675, MLD, Myelin A1 protein, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, OTTHUMP00000163776, OTTHUMP00000174387, OTTHUMP00000174388, SHI, Shiverer, SP
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Myelin Basic Protein Antibody [ARC0535] (A80785) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Myelin Basic Protein Antibody [ARC0535] (A80785)
Western blot analysis of extracts of various cell lines, using Anti-Myelin Basic Protein Antibody [ARC0535] (A80785) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-Myelin Basic Protein Antibody [ARC0535] (A80785) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Myelin Basic Protein Antibody [ARC0535] (A80785)
Immunofluorescence analysis of rat brain using Anti-Myelin Basic Protein Antibody [ARC0535] (A80785) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Myelin Basic Protein Antibody [ARC0535] (A80785) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Myelin Basic Protein Antibody [ARC0535] (A80785)
Immunofluorescence analysis of mouse brain using Anti-Myelin Basic Protein Antibody [ARC0535] (A80785) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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