Western Blot - Anti-Myelin Basic Protein Antibody (A13581)
Western blot analysis of extracts of various cell lines, using Anti-Myelin Basic Protein Antibody (A13581) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Myelin Basic Protein Antibody (A13581)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Myelin Basic Protein Antibody (A13581) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Myelin Basic Protein Antibody (A13581)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Myelin Basic Protein Antibody (A13581) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Myelin Basic Protein Antibody (A13581)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Myelin Basic Protein Antibody (A13581) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Myelin Basic Protein Antibody (A13581)
Immunofluorescence analysis of mouse brain cells using Anti-Myelin Basic Protein Antibody (A13581) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Myelin Basic Protein Antibody (A13581)
Immunofluorescence analysis of rat brain cells using Anti-Myelin Basic Protein Antibody (A13581) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).