Anti-MyD88 Antibody (A82548)

Name: Anti-MyD88 Antibody
Brand: Antibodies.com
SKU: A82548
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to MyD88 for ELISA, WB, IHC, IF and Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Biotin

Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-MyD88 Antibody

Description

Goat polyclonal antibody to MyD88.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 0.3-0.5µg/ml, IHC: 4-6µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human MyD88.

Sequence

C-IKYKAMKKEFP

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

28 kDa

Predicted MW

28.3kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Mutant myeloid differentiation primary response 88, MYD 88, MYD88D, MYD88_HUMAN, Myeloid differentiation marker 88, Myeloid differentiation primary response 88, Myeloid differentiation primary response gene, Myeloid differentiation primary response gene (88), Myeloid differentiation primary response gene 88, Myeloid differentiation primary response protein MyD88, OTTHUMP00000161718, OTTHUMP00000208595, OTTHUMP00000209058, OTTHUMP00000209059, OTTHUMP00000209060

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-MyD88 Antibody (A82548)

MyD88 expression in Human Spleen lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-MyD88 Antibody (A82548) at 0.5µg/ml and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-MyD88 Antibody (A82548)

MyD88 expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-MyD88 Antibody (A82548) at 6µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 3: Immunohistochemistry - Anti-MyD88 Antibody (A82548)

Negative control for MyD88 expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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Figure 4: Immunofluorescence - Anti-MyD88 Antibody (A82548)

MyD88 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-MyD88 Antibody (A82548) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.