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Anti-Myc Tag Antibody [ARC5004-12] (A305783)

Western Blot - Anti-Myc Tag Antibody [ARC5004-12] (A305783) - Antibodies.com Western Blot - Anti-Myc Tag Antibody [ARC5004-12] (A305783) - Antibodies.com Immunofluorescence - Anti-Myc Tag Antibody [ARC5004-12] (A305783) - Antibodies.com Western Blot - Anti-Myc Tag Antibody [ARC5004-12] (A305783) - Antibodies.com
$335
Product Datasheet

Rabbit monoclonal [ARC5004-12] antibody to Myc Tag for WB, ICC/IF and IP.

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Mouse monoclonal [9E10] antibody to Myc Tag for Flow Cytometry, IP, WB, IHC-P and Flow Cytometry (Intracellular).
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Anti-Myc Tag Antibody [MYC.A7]
Mouse monoclonal (MYCA7) antibody to Myc Tag for Dot, ELISA, IP, IS and WB.
Name
Anti-Myc Tag Antibody [ARC5004-12]
Description
Rabbit monoclonal [ARC5004-12] antibody to Myc Tag.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:10,000-1:140,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000
Reactivity
Species Independent
Immunogen
A synthetic peptide corresponding to Myc tag.
Sequence
EQKLISEEDL
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC5004-12
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
58 kDa (NLK) / 72 kDa (YAP1) / 55 kDa (PTEN)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
avian myelocytomatosis viral oncogene homolog, bHLHe39, c-Myc, c-myc tag, class E basic helix-loop-helix protein 39, MRTL, MYC, Myc Epitope Tag, myc proto-oncogene protein, myc-related translation/localization regulatory factor, oncogene c-Myc, proto-oncogene c-Myc, protooncogene homologous to myelocytomatosis virus, transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Myc Tag Antibody [ARC5004-12] (A305783) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Myc Tag Antibody [ARC5004-12] (A305783)
Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein, using Anti-Myc Tag Antibody [ARC5004-12] (A305783) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-Myc Tag Antibody [ARC5004-12] (A305783) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Myc Tag Antibody [ARC5004-12] (A305783)
Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein and 293T transfected with PTEN Protein, using Anti-Myc Tag Antibody [ARC5004-12] (A305783) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-Myc Tag Antibody [ARC5004-12] (A305783) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Myc Tag Antibody [ARC5004-12] (A305783)
Immunofluorescence analysis of HeLa cells transfected with Myc-Tag and untreated HeLa cells using Anti-Myc Tag Antibody [ARC5004-12] (A305783) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-Myc Tag Antibody [ARC5004-12] (A305783) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Anti-Myc Tag Antibody [ARC5004-12] (A305783)
Immunoprecipitation analysis of 300µg extract cell lysate from 293T cells transfected with NLK expression vector containing a Myc-Tag using 3µg of Anti-Myc Tag Antibody [ARC5004-12] (A305783). This Western blot was performed on the immunoprecipitate using Anti-Myc Tag Antibody [ARC5004-12] (A305783) at 1:5,000 dilution.
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