Recombinant Anti-MVP Antibody [r1032] (A315346)

Anti-MVP Antibody [r1032]

Recombinant mouse monoclonal [r1032] antibody to MVP.

Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

WB, IHC-P

MCF-7 or HeLa cells. Human breast tumors.

WB: 1-2 µg/ml, IHC: 1-2 µg/ml

Human

Proteins precipitated from human breast cancer MCF-7 cells.

Mouse

Monoclonal

r1032

kappa

Unconjugated

Protein A/G chromatography.

200 µg/ml

104-110 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

LRP, Lung resistance related protein, Lung resistance-related protein, Major vault protein, Major vault protein, rat, homolog of, MVP_HUMAN, testicular secretory protein Li 30, VAULT 1, VAULT1