Recombinant mouse monoclonal [rMSA/953] antibody to Muscle Actin.
Specificity
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Applications
WB, IHC-P
Dilutions
WB: 0.5-1 µg/ml, IHC-P: 0.5-2 µg/ml
Reactivity
Human, Rabbit, Rat
Immunogen
Recombinant fragment corresponding to human Muscle Actin. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
rMSA/953
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
42 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Muscle Actin Antibody [rMSA/953] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Figure 2: Western Blot - Anti-Muscle Actin Antibody [rMSA/953] (A277943)
Western blot analysis of human skeletal muscle tissue lysate using Anti-Muscle Actin Antibody [rMSA/953].
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