Recombinant Anti-Mucin 5AC Antibody [MUC5AC/7066R] (A278031)

Anti-Mucin 5AC Antibody [MUC5AC/7066R]

Recombinant rabbit monoclonal [MUC5AC/7066R] antibody to Mucin 5AC.

This antibody recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).Its epitope is located in the N-terminal cysteine rich part of the peptide core of MUC5AC, which is heavily glycosylated. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. MAb MUC5AC/7066R reacts with the protein backbone exclusively; it only reacts with fully deglycosylated MUC5AC. Therefore, the material under test should also be fully deglycosylated. This can be achieved with standard periodate oxidation method. The success of the deglycosylation can be checked with routine PAS (Periodic Acid Shiff) staining. After deglycosylation, the preparation should no longer be stainable with PAS reagent. Only then MUC5AC/7066R will react should MUC5AC be present. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2.Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human

This antibody is predicted to cross react with Monkey, Bovine, Feline, and Mouse.

Recombinant full-length human MUC5AC protein.

Rabbit

Monoclonal

MUC5AC/7066R

Unconjugated

Protein A/G chromatography.

200 µg/ml

>1,000 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Mucin 5AC Antibody [MUC5AC/7066R] - BSA and Azide free (A278619).

Apomucin, Apomucin Major Airway Glycoprotein, Gastric mucin, LeB, Lewis B blood group antigen, Major Airway Glycoprotein, MUC 5, MUC5, MUC5AC, Mucin, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5 subtypes A and C tracheobronchial/gastric, Mucin 5, tracheobronchial/gastric, Mucin 5AC oligomeric mucus/gel forming, Mucin 5AC, oligomeric mucus/gel forming pseudogene, TBM, Tracheobronchial mucin, Tracheobronchial Mucin (TBM)