Principle of Assay
Mouse Wnt8b ELISA Kit (A311441) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Wnt8b in An antibody specific for Wnt8b has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Wnt8b present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Wnt8b Antibody, which also binds the Wnt8b present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Wnt8b Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Wnt8b Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Wnt8b captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Wnt8b in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.