Mouse VEGF R2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse VEGF R2 in the samples. A monoclonal antibody specific for mouse VEGF R2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for VEGF R2, and VEGF R2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of VEGF R2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Cell culture supernatant, serum and plasma.

Sensitivity

3.26 pg/ml

Product Range

31.25 - 2000 pg/ml

Specificity

This kit can assay both natural and recombinant mouse VEGF R2.

Reactivity

Mouse

Cross Reactivity

This assay has shown no significant cross-reactivity.

Recovery

Storage

Store at 2 - 8°C.

Synonyms

CD309, CD309 antigen, EC 2.7.10.1, Fetal liver kinase 1, FLK-1, FLK1, FLK1, mouse, homolog of, Kdr, Kinase insert domain receptor, Kinase insert domain receptor (a type III receptor tyrosine kinase), KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Protein-tyrosine kinase receptor flk-1, soluble VEGFR2, Tyrosine kinase growth factor receptor, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR 2, VEGFR-2, VEGFR2, VGFR2_HUMAN

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.