Mouse TNF alpha ELISA Kit (Small Sample Volume) (A323189)

Mouse TNF alpha ELISA Kit (Small Sample Volume)

Mouse GM-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse GM-CSF in serum, plasma, and other biological fluids. This ELISA kit only requires 25µl of sample volume(s).

Sandwich (quantitative)

Mouse TNF alpha ELISA Kit (A323189) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse TNF alpha in serum, plasma, and other biological fluids. An antibody specific for TNF alpha has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TNF alpha present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-TNF alpha Antibody, which binds the captured TNF alpha present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TNF alpha captured in each well. The concentration of TNF alpha can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

4.69 pg/ml

7.81-500 pg/ml

Mouse

No significant cross-reactivity or interference between mouse TNF alpha and analogues was observed.

Intra-assay CV: < 10%, Inter-assay CV: < 10%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

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