Mouse TNF alpha ELISA Kit (Small Sample Volume) (A323189)

$495
Freight Charges
Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Mouse TNF alpha ELISA Kit (Small Sample Volume)
Description
Mouse GM-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse GM-CSF in serum, plasma, and other biological fluids. This ELISA kit only requires 25µl of sample volume(s).
Assay Type
Sandwich (quantitative)
Principle of Assay
Mouse TNF alpha ELISA Kit (A323189) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse TNF alpha in serum, plasma, and other biological fluids. An antibody specific for TNF alpha has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TNF alpha present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-TNF alpha Antibody, which binds the captured TNF alpha present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TNF alpha captured in each well. The concentration of TNF alpha can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colormetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, and other biological fluids.
Sensitivity
4.69 pg/ml
Product Range
7.81-500 pg/ml
Assay Time
3h 30m
Reactivity
Mouse
Cross Reactivity
No significant cross-reactivity or interference between mouse TNF alpha and analogues was observed.
Recovery
Sample Type n Range Average
Serum 8 100% - 109% 103%
EDTA Plasma 8 92% - 108% 100%
Cell Culture Media 8 87% - 96% 93%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 88% - 100% 99% - 109% 85% - 98% 88% - 96%
EDTA Plasma (n=5) 89% - 102% 88% - 100% 95% - 107% 94% - 105%
Cell Culture Media (n=5) 85% - 97% 86% - 99% 93% - 106% 88% - 98%
Precision
Intra-assay CV: < 10%, Inter-assay CV: < 10%
Storage
Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
Synonyms
APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, soluble form, Tnf, TNF a, TNF macrophage derived, TNF monocyte derived, TNF superfamily member 2 , TNF superfamily, member 2, TNF, macrophage derived, TNF, monocyte derived, TNF-a, TNF-alpha, TNFA, TNFA_HUMAN, TNFSF2, Tumor necrosis factor, Tumor necrosis factor (TNF superfamily member 2) , Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor, Membrane Form, Tumor necrosis factor, soluble form
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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