This TGF beta 2 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-TGF beta 2 Antibody. Standards or samples are then added to the microtire plate wells and TGF beta 2, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of TGF beta 2 present in the sample, Anti-TGF beta 2 Antibody (HRP) is added to each well to "sandwich" the TGF beta 2 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TGF beta 2 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, plasma, urine, cell culture supernatants, and tissue samples.

Product Range

10-800 pg/ml

Reactivity

Mouse

Recovery

Storage

Store at 2-8°C for 6 months.

Synonyms

BSC-1 cell growth inhibitor, BSC1 cell growth inhibitor, Cetermin, G-TSF, Glioblastoma-derived T-cell suppressor factor, GTSF, LAP, Latency-associated peptide, MGC116892, MGF, Milk growth factor, Polyergin, TGF-beta-2, TGF-beta2, TGFB2, TGFB2_HUMAN, Transforming growth factor beta 2

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.