Principle of Assay
The Mouse/Rabbit HRP/DAB Kit can simultaneously detect both mouse and rabbit antibodies. This System is suitable for use with mouse IgG, IgM and rabbit primary antibodies, both monoclonal and polyclonal. This Detection System can be used for manual or automated staining. The increased sensitivity of the Mouse/Rabbit HRP/DAB Kit enables faster staining procedures without compromising results. The user may need to further dilute primary antibody due to the superior sensitivity of the Mouse/Rabbit HRP/DAB Kit. The Mouse/Rabbit HRP/DAB Kit is a non-biotin, two-step detection system suitable for labeling antigens in formalin-fixed paraffin-embedded tissues and cryostat sections. The Mouse/Rabbit HRP/DAB Kit may also be used with blood smears, cytosmears, and cell preparations. This System has been developed by directly labeling immunoglobulins with enzymes using a proprietary tandem hyperlabelling technology. This ensures consistent and reproducible immunostaining for all types of nuclear, cytoplasmic and membrane antigens in different types of tissues with significantly lower background than detection systems using biotin and avidin conjugates.
