This M-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-M-CSF Antibody. Standards or samples are then added to the microtire plate wells and M-CSF, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of M-CSF present in the sample, Anti-M-CSF Antibody (Biotin) is added to each well to "sandwich" the M-CSF immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain M-CSF will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensitivity

< 1 pg/ml

Product Range

15.6-1000 pg/ml

Reactivity

Mouse

Recovery

Storage

Store at 2-8°C for 4 months or at -20°C for 8 months.

Synonyms

Colony stimulating factor 1, Colony stimulating factor 1 (macrophage), Colony stimulating factor macrophage specific, CSF 1, CSF-1, CSF1, CSF1_HUMAN, Csfm, Lanimostim, M CSF, Macrophage colony stimulating factor, Macrophage Colony Stimulating Factor 1, MCSF, MGC31930, Processed macrophage colony-stimulating factor 1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.