Mouse IL-17F ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-17F in the samples. A monoclonal antibody specific for mouse IL-17F has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-17F, and IL-17F present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-17F bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.59 pg/ml
Product Range
31.25 - 2000 pg/ml
Specificity
This kit can assay both natural and recombinant mouse IL-17F.
Reactivity
Mouse
Cross Reactivity
This assay has shown no significant cross-reactivity.
Recovery
106% (Average Recovery from Serum Samples).
Storage
Store at 2 - 8°C.
Synonyms
CANDF6, Cytokine ML 1, Cytokine ML-1, IL 17F, IL 24, IL-24, IL17F, IL17F_HUMAN, Interleukin 17F, Interleukin 24, Interleukin-17F, Interleukin-24, ML 1, ML1, Mutant IL 17F, OTTHUMP00000016602
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
