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Recombinant Anti-Mouse IgG2c Antibody [RM223] (Biotin) (A121266)

ELISA - Anti-Mouse IgG2c Antibody (Biotin) [RM223] (A121266) - Antibodies.com ELISA - Anti-Mouse IgG2c Antibody (Biotin) [RM223] (A121334) - Antibodies.com Western Blot - Anti-Mouse IgG2c Antibody (Biotin) [RM223] (A121266) - Antibodies.com
$480

Recombinant rabbit monoclonal [RM223] antibody to Mouse IgG2c (Biotin) for IP, ICC, IHC, Flow Cytometry, ELISA and WB (non-reduced).

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-Mouse IgG2c Antibody [RM223] (Biotin)
Description
Recombinant rabbit monoclonal [RM223] antibody to Mouse IgG2c (Biotin).
Host Species
Rabbit
Target Species
Mouse
Specificity
This antibody recognizes the Fc region of mouse IgG2c.
Applications
IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced)
Recommended Dilutions
ELISA: 0.01-0.2 µg/ml, ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml, WB: 0.5-2 µg/ml
Immunogen
Mouse IgG2c.
Antigen Species
Mouse
Clonality
Monoclonal
Isotype
IgG
Conjugate

Biotin

Product Form
Liquid
Concentration
1 mg/ml
Purification
Protein A chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA - Anti-Mouse IgG2c Antibody (Biotin) [RM223] (A121266) - Antibodies.comEnlarge Image
Figure 1: Anti-Mouse IgG2c Antibody [RM223] (Biotin) (A121266)
ELISA of IgG from BalB/C and C57BL6 shows Anti-Mouse IgG2c Antibody [RM223] reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgG2c Antibody [RM223] was used as the primaryantibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-Mouse IgG2c Antibody (Biotin) [RM223] (A121334) - Antibodies.comEnlarge Image
Figure 2: Anti-Mouse IgG2c Antibody [RM223] (Biotin) (A121266)
A titer ELISA of mouse IgG2c. The plate was coated with different amounts of mouse IgG2c. A serial dilution of Anti-Mouse IgG2c Antibody [RM223] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Western Blot - Anti-Mouse IgG2c Antibody (Biotin) [RM223] (A121266) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-Mouse IgG2c Antibody [RM223] (Biotin) (A121266)
Western blot of non-reduced (-) and reduced (+) mouse IgG2c, using 0.5µg/ml of Anti-Mouse IgG2c Antibody [RM223] (Biotin). This antibody reacts to non-reduced IgG2c.
Publishing research using Recombinant Anti-Mouse IgG2c Antibody [RM223] (Biotin) (A121266)? Please let us know so that we can list the citation on this page.
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