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Recombinant Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin) (A121262)

Western Blot - Anti-Mouse IgG2a Kappa Antibody (Biotin) [RM107] (A121262) - Antibodies.com ELISA - Anti-Mouse IgG2a Kappa Antibody (Biotin) [RM107] (A121262) - Antibodies.com ELISA - Anti-Mouse IgG2a Kappa Antibody (Biotin) [RM107] (A121330) - Antibodies.com
$480

Recombinant rabbit monoclonal [RM107] antibody to Mouse IgG2a Kappa (Biotin) for IP, ICC, IHC, Flow Cytometry, ELISA and WB (non-reduced).

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin)
Description
Recombinant rabbit monoclonal [RM107] antibody to Mouse IgG2a Kappa (Biotin).
Host Species
Rabbit
Target Species
Mouse
Specificity
This antibody recognizes the Fab region of mouse IgG2a, kappa.
Applications
IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced)
Recommended Dilutions
ELISA: 0.005-0.2 µg/ml, ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml, WB: 0.10-5 µg/ml
Immunogen
Mouse IgG.
Antigen Species
Mouse
Clonality
Monoclonal
Isotype
IgG
Conjugate

Biotin

Product Form
Liquid
Concentration
1 mg/ml
Purification
Protein A chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Mouse IgG2a Kappa Antibody (Biotin) [RM107] (A121262) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin) (A121262)
Western blot of non-reduced (-) and reduced (+) mouse IgG2a? (20ng/lane), using 0.2µg/ml of Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin). This antibody only reacts to non-reduced Mouse IgG2a?.
ELISA - Anti-Mouse IgG2a Kappa Antibody (Biotin) [RM107] (A121262) - Antibodies.comEnlarge Image
Figure 2: Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin) (A121262)
ELISA of mouse immunoglobulins shows Anti-Mouse IgG2a Kappa Antibody [RM107] reacts to the Fab region of mouse IgG2a?. There is no cross reactivity with IgG2a?, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgG2a Kappa Antibody [RM107] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-Mouse IgG2a Kappa Antibody (Biotin) [RM107] (A121330) - Antibodies.comEnlarge Image
Figure 3: Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin) (A121262)
A titer ELISA of mouse IgG2a?. The plate was coated with different amounts of mouse IgG2a?. A serial dilution of Anti-Mouse IgG2a Kappa Antibody [RM107] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Publishing research using Recombinant Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin) (A121262)? Please let us know so that we can list the citation on this page.
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