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Recombinant Anti-Mouse IgG1 Antibody [RM106] (Biotin) (A121284)

Western Blot - Anti-Mouse IgG1 Antibody (Biotin) [RM106] (A121284) - Antibodies.com ELISA - Anti-Mouse IgG1 Antibody (Biotin) [RM106] (A121284) - Antibodies.com ELISA - Anti-Mouse IgG1 Antibody (Biotin) [RM106] (A121356) - Antibodies.com
$480

Recombinant rabbit monoclonal [RM106] antibody to Mouse IgG1 (Biotin) for ELISA, WB and IHC.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-Mouse IgG1 Antibody [RM106] (Biotin)
Description
Recombinant rabbit monoclonal [RM106] antibody to Mouse IgG1 (Biotin).
Host Species
Rabbit
Target Species
Mouse
Specificity
This antibody recognizes the Fc region of mouse IgG1.
Applications
ELISA, WB, IHC
Recommended Dilutions
ELISA: 0.005-2 µg/ml, ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml, WB: 0.1-0.5 µg/ml
Immunogen
Mouse IgG.
Antigen Species
Mouse
Clonality
Monoclonal
Isotype
IgG
Conjugate

Biotin

Product Form
Liquid
Concentration
1 mg/ml
Purification
Protein A chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Mouse IgG1 Antibody (Biotin) [RM106] (A121284) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Mouse IgG1 Antibody [RM106] (Biotin) (A121284)
Western blot of non-reduced (-) and reduced (+) mouse IgG1 (20ng/lane), using 0.2µg/ml of Anti-Mouse IgG1 Antibody [RM106] (Biotin). This antibody reacts to non-reduced IgG1 (~150 kDa) stronger than the reduced ?1 form (~50 kDa).
ELISA - Anti-Mouse IgG1 Antibody (Biotin) [RM106] (A121284) - Antibodies.comEnlarge Image
Figure 2: Anti-Mouse IgG1 Antibody [RM106] (Biotin) (A121284)
ELISA of mouse immunoglobulins shows Anti-Mouse IgG1 Antibody [RM106] reacts to the Fc region of mouse IgG1. There is no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgG1 Antibody [RM106] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-Mouse IgG1 Antibody (Biotin) [RM106] (A121356) - Antibodies.comEnlarge Image
Figure 3: Anti-Mouse IgG1 Antibody [RM106] (Biotin) (A121284)
A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of Anti-Mouse IgG1 Antibody [RM106] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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