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Recombinant Anti-Mouse IgG Fc Antibody [RMG06] (Biotin) (A121258)

ELISA - Anti-Mouse IgG Fc Antibody (Biotin) [RMG06] (A121328) - Antibodies.com ELISA - Anti-Mouse IgG Fc Antibody (Biotin) [RMG06] (A121258) - Antibodies.com
$525

Recombinant goat monoclonal [RMG06] antibody to Mouse IgG Fc (Biotin) for IP, ICC, IHC, Flow Cytometry and ELISA.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-Mouse IgG Fc Antibody [RMG06] (Biotin)
Description
Recombinant goat monoclonal [RMG06] antibody to Mouse IgG Fc (Biotin).
Host Species
Goat
Target Species
Mouse
Specificity
This antibody recognizes the Fc region of mouse IgG, including: IgG1, IgG2a, and IgG2b. It reacts weakly with mouse IgG3.
Applications
IP, ICC, IHC, Flow Cytometry, ELISA
Recommended Dilutions
ELISA: 0.05-1 µg/ml, ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml
Immunogen
Mouse IgG.
Antigen Species
Mouse
Clonality
Monoclonal
Isotype
IgG
Conjugate

Biotin

Product Form
Liquid
Concentration
1 mg/ml
Purification
Protein G chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA - Anti-Mouse IgG Fc Antibody (Biotin) [RMG06] (A121328) - Antibodies.comEnlarge Image
Figure 1: Anti-Mouse IgG Fc Antibody [RMG06] (Biotin) (A121258)
ELISA of mouse immunoglobulins shows Anti-Mouse IgG Fc Antibody [RMG06] reacts to the Fc region of mouse IgG1, IgG2a, and IgG2b, and very slightly to IgG3. There is no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50ng/well of different immunoglobulins. 500ng/ml, 200ng/ml, or 50ng/ml of Anti-Mouse IgG Fc Antibody [RMG06] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
ELISA - Anti-Mouse IgG Fc Antibody (Biotin) [RMG06] (A121258) - Antibodies.comEnlarge Image
Figure 2: Anti-Mouse IgG Fc Antibody [RMG06] (Biotin) (A121258)
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of Anti-Mouse IgG Fc Antibody [RMG06] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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