Mouse HSP90 beta ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse HSP90 beta in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Assay Type

Sandwich (quantitative)

Principle of Assay

Mouse HSP90 beta ELISA Kit (A326156) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse HSP90 beta in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. An antibody specific for HSP90 beta has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HSP90 beta present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HSP90 beta Antibody, which binds the captured HSP90 beta present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HSP90 beta captured in each well. The concentration of HSP90 beta can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Platform

Pre-coated Microplate (12 x 8 well strips)

Detection Type

Colourmetric

Instrument

Colorimetric Microplate Reader

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Sensitivity

0.56 ng/ml

Product Range

1.56-100 ng/ml

Assay Time

4h 30m

Reactivity

Mouse

Recovery

Precision

Intra-assay: CV < 10%, Inter-assay: CV < 12%

General Notes

Information online is representative. Always refer to the Product Manual inside the kit.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Synonyms

90 kda heat shock protein beta HSP90 beta, D6S182, FLJ26984, Heat shock 84 kDa, Heat shock 90kD protein 1, beta, Heat shock 90kDa protein 1 beta, Heat shock protein 90 alpha family class B member 1, Heat shock protein 90 kDa, Heat shock protein 90kDa alpha (cytosolic) class B member 1, Heat shock protein 90kDa alpha family class B member 1, Heat shock protein beta, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta, HS90B_HUMAN, HSP 84, HSP 90, HSP 90 b, HSP 90b, HSP84, hsp90ab1, HSP90B, HSPC2, HSPCB

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.