Mouse GM-CSF ELISA Kit (A579)

$635
Freight Charges
Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Mouse GM-CSF ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This GM-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-GM-CSF Antibody. Standards or samples are then added to the microtire plate wells and GM-CSF, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of GM-CSF present in the sample, Anti-GM-CSF Antibody (Biotin) is added to each well to "sandwich" the GM-CSF immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GM-CSF will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensitivity
< 1 pg/ml
Product Range
15.6-1000 pg/ml
Reactivity
Mouse
Recovery
Storage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
Colony stimulating factor, Colony Stimulating Factor 2, Colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, CSF 2, CSF2, CSF2_HUMAN, GMCSF, Granulocyte Macrophage Colony Stimulating Factor, Granulocyte-macrophage colony-stimulating factor, MGC131935, MGC138897, MGI1GM, Molgramostin, Pluripoietin-a, Sargramostim
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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