This Galectin 1 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Galectin 1 Antibody. Standards or samples are then added to the microtire plate wells and Galectin 1, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Galectin 1 present in the sample, Anti-Galectin 1 Antibody (Biotin) is added to each well to "sandwich" the Galectin 1 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Galectin 1 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensitivity

< 5 pg/ml

Product Range

156-10,000 pg/ml

Reactivity

Mouse

Recovery

Storage

Store at 2-8°C for 4 months or at -20°C for 8 months.

Synonyms

14 kDa laminin-binding protein, 14 kDa lectin, Beta galactoside binding lectin, Beta galactoside binding lectin L 14 I, beta galactoside binding protein, Beta-galactoside-binding lectin L-14-I, DKFZp686E23103, Gal 1, Gal-1, GAL1, Galaptin, Galbp, Galectin, Galectin-1, Galectin1, GBP, HBL, HLBP14, HPL, L 14.5, L-14.5, L14, Lactose binding lectin 1, Lactose-binding lectin 1, Lect14, Lectin galactoside binding soluble 1, Lectin galactoside-binding soluble 1, LEG1_HUMAN, LGALS 1, LGALS1, Lgals1 lectin galactose binding soluble 1, MAPK activating protein MP12, Putative MAPK activating protein MP12, Putative MAPK-activating protein PM12, S Lac lectin 1, S-Lac lectin 1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.