This CXCL1 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-CXCL1 Antibody. Standards or samples are then added to the microtire plate wells and CXCL1, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of CXCL1 present in the sample, Anti-CXCL1 Antibody (Biotin) is added to each well to "sandwich" the CXCL1 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CXCL1 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensitivity

< 1 pg/ml

Product Range

15.6-1000 pg/ml

Reactivity

Mouse

Recovery

Storage

Store at 2-8°C for 4 months or at -20°C for 8 months.

Synonyms

C-X-C motif chemokine 1, chemokine (C-X-C motif) ligand 1, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), CINC-1, Cytokine-induced neutrophil chemoattractant 1, fibroblast secretory protein, FSP, GRO, Gro 1, Gro A, GRO alpha, GRO protein, alpha, GRO-alpha(1-73), GRO-alpha(6-73), GRO1, GRO1 oncogene, GRO1 oncogene (melanoma growth stimulating activity, alpha), GRO1 oncogene (melanoma growth-stimulating activity), GROa, GROA_HUMAN, growth-regulated alpha protein, KC, KC chemokine, mouse, homolog of, Keratinocyte-derived chemokine, mouse, homolog of, Melanoma growth stimulatory activity, melanoma growth stimulatory activity alpha, Melanoma growth stimulatory activity, alpha, MGSA, MGSA a, MGSA alpha, MGSA-a, N51, NAP 3, NAP-3, NAP3, Neutrophil-activating protein 3, Platelet-derived growth factor-inducible protein KC, Scyb 1, SCYB1, Secretory protein N51, Small inducible cytokine subfamily B, member 1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.